Tissue decellularization produces a three-dimensional scaffold that can be used to fabricate
functional liver grafts following recellularization. Inappropriate cell distribution and clotting
during blood perfusion hinder the practical use of recellularized livers. Here we aimed to
establish a seeding method for the optimal distribution of parenchymal and endothelial cells,
and to evaluate the effect of liver sinusoidal endothelial cells (LSECs) in the decellularized
liver. Primary rat hepatocytes and LSECs were seeded into decellularized whole-liver …